We tested a potential anti-cancer treatment in balb/c mice injected with 4T1 cells (25 animals). We collected blood initially, at D7, D14, D21 and D28; We also collected supernatant of spleen cells placed for 24 hr in the incubator at the time of euthanasia. Very little is known about the mechanism of this treatment.
We looked at the expression of 25 cytokines using 25-plex assay; I now need to analyze the data with very little knowledge in immunology, and a lot of variation between samples.
Do you know of a good tool that can integrate data considering multiple cytokines to identify mechanism/ pathway? Or do you have any recommendations/advice?
Thank you!
Hi, please use multiplex ELISA kits. You can order from Elabscience company.
Hi Emmanuel,
I used a multiplex kit from Luminex, already have the data. I am now trying to make sense of the variations in cytokine levels I see between groups and over the time and am looking for immunology/bioinformatics tools to help integrate the data.
I guess you can do a heat map analysis. I've see quite a few publications that uses this method. Someone one another thread talked about it. (I don't do this. So I can't give you an idea of the type of programs to use.)
https://www.researchgate.net/post/Can_anyone_suggest_another_software_besides_R_for_heatmap_for_cytokine_data
But to make sense of the heat map analysis, you still need to have knowledge of immunology.
Thank you Shen-An. I am in the process of educating myself, reading published literature. Tedious, but necessary!
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