I have been looking into specific markers to detect memory T cells, as there is not just one unique marker to detect them, I was wondering which would be a good combination, would CD45RO/CD3 work?
Hi Almudena.
The better way to detect memory T cells is to stain them with CD3, CD4, CD45RA, CD45RO to be sure to have only memory cells
Memory T cells are : CD3+/CD4+/CD45RA-/CD45RO+.
Good luck.
Hi Almudena,
Memory T cells are quite heterogeneous...
I would use CD3, CD197, CD45RA and CD95 as a minimal panel. This allow you to discriminate Naïve cells (CD197+ CD45RA+ CD95-) and memory subsets: TSCM (memory stem T cells that are CD197+ CD45RA+ CD95+), TCM (central memory T cells : CD197+ CD45RA-), TEM (effector memory T cells : CD197- CD45RA-) and TEffD (terminally differentiated effector T cells : CD197- CD45RA+). Please note that other discretes subsets exist as well as other possible markers (eg CD27, CD62L, CD57, CD28...).
As always, it depends on the answers you are looking for!
Hope it helps.
Marcelo
Hey Almudena,
I think you are working with human cells?
If so, we used to work with T cells from peripheral blood. As far as I can tell, it is relatively well accepted to use CD27 and CD45RA:
Memory T cells: CD27+CD45RA-
Effector (or effector memory) T cells: CD27-CD45RA-
Naive T cells: CD27+CD45RA+
TEMRA: CD45RA+ CD27-
If you look in the papers, you might find there is debate about how accurate these classification is and I do think there's heterogeneity within these subsets.
In practical terms, I'd say go ahead with those markers.
If you think you need to get more nuanced, this reference might be a good way to get you thinking about the issue.
https://www.nature.com/articles/nm0201_245
Good luck!
Hi Sergio:
thank you for your suggestions, anyhow as I also work with mouse tissues, i was wondering if it is very different?
Almudena
Yep. It is pretty rough to classify memory T cells solely based on surface markers, because of heterogeneity. Antigen-specific would be better but that's not always feasible.
For mouse, CD44 and CD62L are used widely. If you are working in mouse tissues, you might want to add CD69 and CD103 for looking at TRM cells
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