Immunology

Immunology is a broad branch of biomedical science that covers the study of all aspects of the immune system in all organisms. It deals with the... | Contact experts in Immunology to get answers

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Questions related to Immunology

How to determine if a drc model is statistically fitting my data ?

Dear community, I am facing some issues and scientific questionings regarding the dose-response analysis using the drc package from R. Context : I want to know if two strains have different...

01 February 2021 8,241 1 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

13 January 2021 3,802 2 View

Cell number for 1mg protein?

We need to isolate 1mg protein, from ipsc-derived cortical neurons. About how many cells should I have, to get this protein amount from these cells?

18 December 2020 3,104 1 View

Longitudinal Data on Well-Being?

Do you know an available (open access) longitudinal data on well-being (e.g. National Survey of Mental Health and Well-Being)? I want to learn and study the data to help me build a growth model.

10 November 2020 1,851 3 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

27 October 2020 6,196 4 View

How to successfully produce agarose microwells with PDMS stamp?

My experiment aims to make spheroids by sacrificial micromolding technique. I am following the easy protocol: "...The PDMS stamps were deposited onto a 250 μl drop of 3% liquid agarose within the...

06 October 2020 3,863 3 View

Does anyone know the exact cleavage site of SaCas9?

Spcas9 has been reported to cleave ~ 3bp upstream of the start of the PAM as a blunt cut, Cpf1 has a staggered cut significantly upstream of the PAM, but I cant seem to find much information...

30 September 2020 9,079 2 View

Do the tannic acid-treated sheep RBCs show agglutination with FAdVs (fowl adenoviruses)?

As we know that Adenoviridae indirectly agglutinates the RBCs of chicken, mice, rhesus monkey, and human blood type "O".However, do the tannic acid-treated sheep RBC show agglutination with FAdVs...

18 September 2020 6,819 3 View

Why Salmonella makes TSB (Tryptic Soy Broth) medium get darker?

Hi everyone, we are making some experiments with Salmonella species in a microplate reader. We are basically characterizing its growth curve in 18 hour time of incubation. However, only one...

09 September 2020 2,833 5 View

Can anti-Fas (anti-CD95) be used to prepare apoptotic cancer cell as antigens for DC to present antigen?

Researchers usually used irradiation (such as x-ray) to induce cancer cell apoptosis to prepare available antigens for DC to uptake that present the antigen to T cells. Can other apoptotic methods...

27 August 2020 5,589 4 View

The difference between Ficoll-Paque PLUS and Ficoll-Paque premium in real life practice and research?

Can anyone advise if there is a real difference between Ficoll-Paque PLUS vs Ficoll-Paque PREMIUM regarding the isolated lymphocytes phenotypes or its quality?

15 August 2020 6,683 4 View

Does crosslinking actually increase or decrease the antigenicity of collagen as biomaterial?

Some studies point that crosslinking increase the inflammatory reaction of collagen[1-3]. while some other studies suggests that Cross-linking also can be introduced to reduce the antigenicity....

11 August 2020 3,414 3 View

Can anyone suggest a PhD dissertation area that encompass Microbiology and Immunology?

Can anyone please suggest some related topic or area in microbiology and immunology except COVID/SAARS?

05 July 2020 5,110 37 View

What's your expectation about the successful treatment of COVID-19 in the future, do you think will be immunologically or synthetically by drugs?

The clinical treatment of COVID-19 infected individuals.

25 June 2020 1,658 22 View

In vitro method for allele-specific antibody detection?

I am looking at allelic variation in surface antigens of Plasmodium spp. I'd like to look at antibody responses from these specific alleles of the antigens, however I am trying to avoid the use...

21 June 2020 6,679 0 View

Bioinformatics tools to discover new Biomarkers?

I am interested in the research related to discover novel biomarkers for bacterial (pathogenic) disease. What types of bioinformatics approach and tools can be used for the same? Thanks,

18 June 2020 5,289 5 View

Does LPS elicit an inflammatory response in the neutrophil-like cell line HL-60?

I'm replacing primary neutrophils with the neutrophil-like cell line HL-60 for immunological studies. Do HL-60 cells, differentiated towards the granulocyte phenotype with retinoic acid or DMSO,...

08 June 2020 2,374 3 View

I want to compare the difference of immune system in patient with mild and sever symptom.who should i define the case and control group?

Is it enough to compare people who needed to be hospitalized with people who did not? , or should the age and duration of the illness be considered?

27 May 2020 2,960 2 View

COVID- 19 : Please suggest some paper about Plasma therapy for COVID- 19 ?

FDA has issued guidance to provide recommendations to health care providers and investigators on the administration and study of investigational convalescent plasma collected from individuals who...

16 May 2020 8,862 14 View

Is it compulsory to publish a theoretical discovery which may even challenge 'General Relativity' in standard journal than normal open access journal?

I have prepared the manuscript to publish an important theoretical result in dark energy physics.The content is novel at high profile thinking. But sure that the paper may not be accepted by high...

14 May 2020 4,577 0 View

Looking for immunologist or virologist to help with literature-based study for COVID treatment. Can you help?

On the side, I doing some research (literature-based) on a particular treatment that may help for COVID-19 symptoms but I need someone with a stronger medical knowledge base with expertise in...

30 April 2020 8,269 11 View

Please some paper about Adaptive immunity against viral infection???

The adaptive, or acquired, immune response takes days or even weeks to become established—much longer than the innate response; however, adaptive immunity is more specific to pathogens and has...

27 April 2020 2,389 10 View

Molecular Biology : What is the best simplified book for Molecular Biology Techniques ?

Molecular biology is the study of the molecular underpinnings of the processes of replication, transcription, translation, and cell function. Biochemistry is the study of the chemical substances...

26 April 2020 6,676 5 View

Which book is best for learning Different Biochemical Techniques ?

Biological techniques are methods or procedures that are used to study living things. They include experimental and computational methods, approaches, protocols and tools for biological research.

20 April 2020 4,467 26 View

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