Immunology

Immunology is a broad branch of biomedical science that covers the study of all aspects of the immune system in all organisms. It deals with the... | Contact experts in Immunology to get answers

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Questions related to Immunology

IVIG and aseptic meningitis, is there any relationship between them?

Nowadays IVIG is prescribed in many rheumatologic and immunologic diseases like GBS and SLE. In some texts, aseptic meningitis is one of the rare side effect of IVIG... I want to collect some new...

03 March 2018 7,209 4 View

Is it possible to have similar CDR3 sequence in Naive and Memory B cells?

We know that class switched B cells have similar CDR3 sequence but do Naive B cells have same CDR3 sequence in Memory B cells? If yes why do Naive and Memory B cells have similar CDR3 sequence?...

03 March 2018 5,758 0 View

Bone Marrow derived DCs yield?

Hi all, I am currently struggling to obtain a high number of immature DC from bone marrow progenitors. For example, from 4 tibias and 4 femurs (Balb/c female) I can have around 55x10^6...

03 March 2018 2,583 8 View

How to increase my RG ??

According to your experience , tell me about the ideal ways to improve researcher RG .. I am new member on research gate and I hope to share knowledge and experience to the universe of research...

03 March 2018 3,936 15 View

Does anyone know why I see cytoplasmic signal when I am trying to detect a nuclear protein through immunofluorescence?

I am using Cyclin B1 as a marker for G2 phase in mouse embryonic tissues. However, when I use my anti Cyclin B1 rabbit monoclonal antibody, I only see signal in the cytoplasm of the cells. The...

03 March 2018 3,163 11 View

I am looking for Phd scholarship in the field of medical parasitology ?

I f you have a team of researchers and need one more I will be happy if you joined me.. I am interested in Medical parasitology , immunology and immuno-parasitology.

03 March 2018 7,091 0 View

What are the specific immunological marker available as kit in lab animals ?

lab expermint

03 March 2018 4,947 7 View

Why use Retinoic Acid to induce differentiation in SH-SY5Y cells?

I have been searching for information on how to induce differentiation in SH-SY5Y cells (epithelial to neuronal), and have discovered several chemical agents that can achieve this. Out of all the...

03 March 2018 773 6 View

Antibody trigger human serium activation?

Hi, I have one immunology question. I am trying to induce serium activation by antibodies. Do I need to use heat to make antibody aggregation beofre adding? Can I just add high conc. antibodies to...

03 March 2018 3,928 0 View

What are the classic immunology books that university students should look at?

Currently I am reading the principles of immunology and history of science

12 February 2018 5,289 9 View

Do you have photograph Passiflora incarnata?

Dear, do you have someone photograph Passiflora incarnata in print quality?

05 February 2018 9,367 1 View

Is anyone aware of immunological changes in the last year of life?

Many studies have demonstrated that frailty, loss of independence and a large increase in hospital admissions are associated with the last year of life. I am looking for any evidence of immune...

02 February 2018 6,537 12 View

What are the classic immunology books that university students should look at?

Currently I am reading the principles of immunology and history of science

02 February 2018 5,763 6 View

Cross-reactivity problem with KLH?

I was going to order short peptides 20-30AA conjugated to KLH to immunize a rabbit for antibodies against a plant pathogen. Fortunately i stumbled across this article which reports KLH-antibodies...

02 February 2018 1,922 4 View

Is limited dilution analysis (LDA) a relevant and trustworthy method?

I got used to cytometric analysis performed on fresh blood. Recently, I read an old article, where LDA was used. Authors stimulated samples with either rIL-2 or PHA and thereafter performed...

23 January 2018 6,044 3 View

Antibody for cell membrane staining for immunofluorescence?

I am looking for antibody against human cell membrane which will circularly stain the cell membrane only. Could anyone name such antibody? I want to avoid F-actin, microtubulin bcz they stain all...

23 January 2018 5,190 11 View

Could anyone help me in vitro cross presentation assay (B3Z assay)?

In my experiment, I treated BMDC (50 000 cells/well) with different concentrations of adjuvant with or without OVA protein ( 80 ug/ml) and incubate them for 4 hours. After washing with PBS, I...

07 January 2018 8,394 4 View

For veterinary medical students, which module must come first, immunology or microbiology?

it was discussed during curriculum development

01 January 2018 3,254 2 View

What are the acute and long term effects of chemoradiation in paediatric cancer patients' haematological and immunological parameters?

Blood paramters

01 January 2018 2,126 2 View

Could anyone help me in vitro cross presentation assay (B3Z assay)?

In my experiment, I treated BMDC (50 000 cells/well) with different concentrations of adjuvant with or without OVA protein ( 80 ug/ml) and incubate them for 4 hours. After washing with PBS, I...

01 January 2018 214 5 View

Does the immune system function in brain dead individuals who are maintained on life-support?

It appears that there is a lack of knowledge regarding the function of the immune system in brain dead individuals who are maintained on life support. I am interested in understanding whether the...

01 January 2018 1,835 4 View

How to improve intracellular cytokine staining for IFN-gamma in human PBMCs?

How to improve intracellular cytokine staining for IFN-gamma in human PBMCs? Actually, I incubate cells with 50ng/ml PMA and 1ug/ml ionomycin for 5 hours. At the same time, we add Golgi stop and...

31 December 2017 5,726 13 View

Can I actually induce memory of T cell in PBMC by doing primary stimulation, resting and restimulation?

Hello, I am new to Immunology. I tried to induce the memory of T cell in PBMC(10%human serum in RPMI) by doing primary antigenic stimulation for 48hrs or 72hrs, and then rest them for 2,4, 6 days...

12 December 2017 1,764 10 View

Experienced researchers familiar with flow cytometry?

Please take a look at the attached files: I am about to study 2 markers which appear in FL1 and FL2; In the unstain tube, I see 2 distinct populations one of which has weaker auto-fluorescence...

11 November 2017 6,552 7 View

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