Immunology

Immunology is a broad branch of biomedical science that covers the study of all aspects of the immune system in all organisms. It deals with the... | Contact experts in Immunology to get answers

2,015 views 2,404 posts

Questions related to Immunology

Stability & storage of salivary alpha amylase?

I'm designing a new activity for an undergraduate biology course about enzyme activity. I'm running into a slight complication. I've ordered a lyophilized powdered version of human salivary...

13 July 2023 653 6 View

Any one can help me to explain why I am geting low purity and concentration of RNA using RNeasy mini and midi kits?

I did several experiments with different amounts of starting material from a Bactria grown on minimal media and a biofilm washed and re-suspended for pelleting by PBS. Mainly the 260/230 ratio is...

04 July 2023 3,299 2 View

What could be the cause of the high A260/A280 ratio (2.48) and the very low-strength 28s and 18s bands observed in the agarose 1% gel electrophoresis?

I am currently facing a challenge related to the extraction of total RNA from 40 mg colon tissue, and I would greatly appreciate your expertise and insights in troubleshooting the issue. During...

20 June 2023 9,500 3 View

How to clean DNA samples contaminated by RNA (details below)?

I am working on some grape Vitis arizonica leaf extractions and after extraction they look great on nanodrop with 260/280 between 1.72 and 2.05 for all samples and 260/230 between 1.7 and 2.10 for...

14 June 2023 6,814 4 View

Study on ChatGPT Usage. How Does Using ChatGPT Impact Mental Health and Well-being?

Greetings! We are conducting a research on the usage of ChatGPT, and we would greatly appreciate your assistance. The study aims to determine the reasons behind people's use of ChatGPT, its impact...

28 April 2023 9,733 1 View

Does Yoghurt, Cheese and Milk consumption reduce gut gram negative bacteria and toxic plasma bacterial lipopolysaccharides?

The literature indicates that yoghurt contains live bacteria that have major effects on gut dysbiosis, metabolism, insulin resistance and neurodegeneration. Gram negative bacteria release...

24 November 2022 4,196 1 View

Sectioning whole FFPE human brains?

We are currently looking at a heavy duty microtome "POLYCUT" from Gigatome for sectioning a human brain (one hemisphere) embedded in paraffin. Does anybody have experience with this type of...

11 October 2022 6,717 0 View

Can probiotics (eg yoghurt) activate the anti-aging gene Sirtuin 1 with relevance to the treatment of Alzheimer’s disease?

The relevance of the Microbiota-Gut-Brain axis to Alzheimer’s and neurodegenerative diseases needs extensive analysis. The various articles indicate that there are various questions with relevance...

03 August 2022 3,910 3 View

Mutatex Foldx Warning error while doing protein mutation is silico?

Hi everyone, While doing a mutation for a Ubiquitine Ligand against a USP enzyme I came across this error which I couldn't know how to solve. warning:root:foldx exited with error for run DB58!...

10 June 2022 4,169 0 View

Any protist virus database?

Do you know any database for protistan viruses? Thank you!

07 June 2022 1,714 0 View

How to exctract difficult plasmids from bacteria?

Dear All, I have received the pLVX-M-puro plasmid in a bacterial stab and I can´t isolate it. I have tried several times, using fresh Amp-plates and Amp-LB media to first grow the single colonies...

13 April 2022 908 7 View

Is histopaque same as ficoll-paque? Can I use histopaque to isolate PBMC?

Peripheral blood mononuclear cells (PBMC)

01 February 2022 8,065 4 View

How to prepare 0.5% cmc _ carboxy methyl cellulose as vehicle to administer drugs to animals?

Drug ethanolic extract of A.calamus. vehicle CMC.

15 December 2021 6,215 0 View

Culturing monocytes and monocyte-derived macrophages isolated from blood samples - How long do these cells last in culture?

Hi everyone, does anyone know the best condition to isolate and culture monocytes and monocyte-derived macrophages? Many papers suggest DMEM, others RPMI + 10% FBS. I am culturing these cells in...

12 November 2021 8,846 4 View

How can I calculate the theoretical molecular weight of my liposomes ?

I want to compare values obtained with the AF4 method I have, but first how can we easily assess the molecular weight ? I have 3 lipids. I assume, we multiply by their respective masses used...

28 September 2021 2,156 0 View

How to count nanoparticles?

I'm dealing with vesicles (not liposomes, so I dont know the composition and I can't do any assumption of their molarity based on their concentration and hydrodinamic radius), derived from...

17 September 2021 271 4 View

Why my lipid vesicles missing during extrusion?

Hi all, I have tried to do a calcein leakage assay by LUVs that I made (dopc:dopg 1:3, dopc:dopg 3:1, and dopc only). In doing so I dissolved my lipids film in 2 mL of 10mM tris buffer containing...

03 September 2021 6,225 1 View

6-Hydroxydopamine hydrobromide (6-OHDA) preparation and storage experience?

My query is, has anyone worked with the compound, I got a 250 mg bottle from SIGMA. But I will only need about 70mg and planning to store the rest for further use. Should I weigh and resuspend per...

30 June 2021 7,116 1 View

How to prepare sodium phosphate dibasic solution?

To adjust the pH 6 on the estimation of dietary fiber

29 June 2021 8,312 4 View

Looking for an HPLC-UV software ?

I'm looking for a software able to process HPLC-UV chromatograms (espacially baseline correction and peak alignement) in order to integrate the data to statistical analysis.

25 May 2021 1,859 10 View

How to convert Waters raw file to mgf or mzML file format keeping the original charge state information?

Hi, I am using a Waters Select Series Cyclic IMS, and trying to convert MSe raw data (without using ionmobility) to another generic file format. I tried PLGS and MSconvert but they transform the...

29 April 2021 5,768 0 View

Questions about NK cells cultivation?

The NK cell culture method I chose is to add K562 and allogeneic PBMCs to the system, which have been treated with 100ug/ml MMC. Freshly isolated PBMCs are sorted by EasySep™ Human NK Cell...

14 April 2021 1,281 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

03 March 2021 1,758 3 View

Problem removing OG detergent from liposomes with Bio-beads?

Hello, I'm having a problem that I don't know how to solve. I am following a protocol that I did a year ago and now it does not work. I reconstitute the dry lipids (polar lipids from E.coli) in a...

10 February 2021 2,776 2 View

Badges
Science topic