Why does T line disappear very fast in the competitive assay?

10 October 2018 0 6K Report

I performed competitive assay in lateral flow. Ag was conjugated with gold particles and Ab was painted on the membrane. T line showed up right after positive control was loaded, while T line disappeared in 1-2 minutes. Negative is fine that T line could stay for long time. Does anyone know the reason and how to solve this problem? Thanks!

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