Cell number for 1mg protein?

18 December 2020 1 3K Report

We need to isolate 1mg protein, from ipsc-derived cortical neurons. About how many cells should I have, to get this protein amount from these cells?

Alexandr Chernov

Dear Reeteka!

Please You look at this information:

https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/CO014773-Cell-Iso-LysisFract-HB-asm1-x1aNoCrops.pdf

(page 18: Table 1. Average total protein yields from neuronal tissue and primary cells )

Primary Neuronal Cells

Sample Size 106 cells Average Total Protein 0.35 ± 0.01mg, Protein Concentration 0.69 ± 0.02mg/mL

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