Why not use the data analysis system (CDS) from the original software that acquired the HPLC data? This would make it easy to load, adjust and output. The features you mention are usually found in most packages would allow analysis of typical 2D chromatograms.

OpenChrom is a free chromatographic software that can process HPLC-UV and even Mass spec data.

Here is the link to the software :

https://lablicate.com/platform/openchrom

Openchrme is a open source software, it supports data analysis for HPLC- UV, GC- MS and NMR , helps to analyze the SPECTROMETRY & spectroscopy.

Rohith Krishna and Jagapriya Jagapriya thanks for the answer, I have tried to use openchrom but I can't open the files ... Our HPLC-UV is working with Chromeleon, the raw data are in txt form (rt/intensities) and I don't know how to open them with Openchrom ... Maybe one of you could help ?

William Letter thanks for the idea but I can't find in chromeleon the options I'm looking for!

Hello again,

I can suggest you two approaches.

1) Use software similar to MZmine 2 or Proteowizard to convert the .raw files. I am not sure about the results for UV data as it is generally used for mass spec data.

2) Export the data from the chromatogram into the clipboard and paste it in Excel and then save the file as a .csv file. This might work for UV data. Please do try these two out and keep the progress updated.

Thank you ! Helene Esselin

Hi Rohith Krishna , thanks for helping.

I've tried the second option : I can open the chromatogram but the intensity scale doesn't match and the chromatogram is reversed ... My csv file is composed of two columns : one for the retention time and the other for intensities, I don't understand why openchrom can't read it properly ...

Thanks again!

Helene Esselin, It is good to know that the technique worked but I will surely look into this issue with the reversed chromatogram and the wavelength problem. I have a feeling that the software considered the file as a spectrum rather than a chromatogram. I suggest you look into the resources on openchrom website. I will try from my end too.

Thank you!

Hi Rohith Krishna , do you know if there is a way to directly contact openchrom support ? The contact form doens't seem to work and i still can't open my files ... thanks a lot

Dear Helene Esselin , Please check your RG inbox I have sent you a personal message with the contact details. Thank you !

You could also try the package I am developing in R: https://github.com/ethanbass/chromatographR. It doesn't have a parser for chromeleon files, but I could probably write one for you if it's really just a text file?

Update: I ended up adding a parser for the Chromeleon text format in the latest version of chromConverter (github.com/ethanbass/chromConverter).