I purchased ficoll paque after long discussion with technical person. The difference in both is just an extra certification. For the certificate they charge extra money.

Practically the should be no difference in both.

Thanks Jitendra Kumar Shandilya. Any exerimental evidence?

hi

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood. For research use only. Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 years as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003. They constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been optimized for the isolation of mononuclear cells from human peripheal blood, bone marrow, and umbilical cord blood. Ficoll-Paque PREMIUM 1.084 can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats. Ficoll-Paque PREMIUM 1.073 can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).

Ordering information: Both Ficoll-Paque® PLUS and PREMIUM media are supplied ready to use and are stable for at least three years when stored between 4 to 30°C (39 to 86°F) and protected from light. Certificate of Analysis is available.

I agree with shandilya

good luck

Thanks Reza Hadavi. Do you have any evidence or experiments to compare the different products outcome in real samples?