Any protist virus database?

Guillermo Domínguez Huerta @Guillermo-Dominguez-Huerta

07 June 2022 0 2K Report

Do you know any database for protistan viruses?

Thank you!

Badges
Science topic

More Guillermo Domínguez Huerta's questions See All

Can I use Polyjet after its expiration date?

I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?

23 July 2024 8,059 1 View

¿Where to buy the Aspergillus oryzae NSAR1 strain?

Greetings: I am looking to buy/obtain the auxotrophic strain Aspergillus oryzae NSAR1, but I just have found one culture collection from Japan but haven't received any answer. Could you provide...

11 July 2024 6,847 0 View

What does effector:target ratio really means in CAR-T cell therapy?

Regarding CAR-T cell therapy, during co-cultures with different effector:target ratios, when we refer to "effector", does it involve the total T cells? For example, does a ratio of 5:1 mean 5...

28 May 2024 2,757 4 View

How can I calculate the effective dielectric constant of a thin film and substrate?

I am trying to figure out a way of calculating the dielectric constant of a thin film of Yb2O3 on ITO. Assuming that the thickness of the Yb2O3 is known and that it's

05 May 2024 5,989 1 View

What's the proper way to clean lactophenol blue from microscope slides?

Hi, I've been cleaning lactophenol blue from microscope slides using ethanol (70% or 96%) but I don't know if it's okay to mix them with lactophenol blue. I already looked for information in SDS,...

22 April 2024 1,251 1 View

¿Piensas que una persona que ha maltratado psicológicamente y físicamente puede rehabilitarse 100%?

ESTÁN TODAS LAS PERSONAS PREPARADAS PARA UNA REHABILITACIÓN 100% EFICAZ

04 March 2024 3,344 3 View

What's the optimal observation count per category for Machine Learning?

Hello everyone, I'm seeking some advice or references related to the optimal number of observations needed per category within a categorical variable for machine learning projects. I've come...

29 February 2024 9,289 2 View

Some expert using "GRADE" to the assessment of certainty in evidence of systematic reviews?

We are preparing an umbrella review and we need someone who wants to collaborate and perform the assessment of certainty in evidence of systematic reviews using GRADE on the systematic reviews...

20 February 2024 9,244 0 View

What am I doing with AI in campus?

With the rising application in AI (ChatGPT, Dall-e, Tome, etc) in education, for better or for worse, what actions are you taking to improve your research and your teaching using AI...

12 February 2024 3,893 2 View

Has anyone have taken confocal images of apoptotic cells stained with Apotracker Tetra?

Hi! I was wondering if anyone has taken confocal images of apoptotic cells stained with Apotracker Tetra, from Biolegend. There is information using Apotracker Green, but I haven´t found a...

06 February 2024 8,343 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

Intracerebroventricular (ICV) AAV inj.?

I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...

12 July 2024 781 0 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

Does anyone know how to improve calcium-imaging fluorescence with GRIN lenses?

Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...

08 July 2024 5,178 2 View

Why do I have two different bands from the same virus after PCR?

I get two different bands from BCMV after PCR

07 July 2024 2,112 1 View

Problems to find a region of a Virus gene?

We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...

01 July 2024 7,644 3 View

I need this article, can anyone get it?

I need this complete article, could anyone help me? follow theme below. Chikungunya virus cell-to-cell transmission is mediated by intercellular extensions in vitro and in vivo.

20 June 2024 3,373 1 View

Can anybody recognize the virus on potato plants?

Infected potato plant was negative for PVY, PVX, PVM, PVS. What can it be?

19 June 2024 6,739 3 View

Protocol for Antibody Dissociation from Virus in Serum Samples?

Hello everyone, I'm currently working on a project where I need to isolate viruses in culture from incubation with PCR-positive serum samples, but I'm facing the challenge of dissociating...

17 June 2024 1,855 1 View