I expressed my protein in Arctic DE3 cells fused with Sumo-tag. I am using 20% Glycerol in the lysate and elution. I seem to have majority of the protein eluting as oligomer. I have 4 di-sulfide bond interactions that I think are important. I also have a couple of n-linked glycosylation cites which I am not sure how important they are. Any suggestions how to de-oligomerize without disrupting the integrity of the protein?

More Sharoon Akhtar's questions See All
Similar questions and discussions