I'm dealing with vesicles (not liposomes, so I dont know the composition and I can't do any assumption of their molarity based on their concentration and hydrodinamic radius), derived from erythrocytes. I would like to count them. I dont have access nor to ZetaView for Nanoparticle tracking analysis or to a resonant mass measurement equipment. We just have a Zetapals, which also counts particle/seconds but I'm not sure about reliability.
I was thinking of some fluorescence method... Do you have any suggestion?
Dear Giulia Della Pelle
The article below presents simple mathematical equations to count particle numbers of different types:
Mozafari, M.R.; Mazaheri, E.; Dormiani, K. Simple Equations Pertaining to the Particle Number and Surface Area of Metallic, Polymeric, Lipidic and Vesicular Nanocarriers. Sci. Pharm. 2021, 89, 15. https://doi.org/10.3390/scipharm89020015
You just need to know how much material (ingredients) you took to make your vesicles and have idea about average particle size. The equations will give you:
NUMBER OF PARTICLES PER MILLILITER.
let me know if you need clarifications.
Dear M. R. Mozafari ,
I already knew your article, and I read it with much pleasure. Unfortunaly, I'm working with a formulation of unknown composition - it's from erythrocytes membrane, which has, rather than being composed by proteins and lipids, asymmetrical distribution of phospholipids between the two leafleats and therefore it's impossibile to know for sure the heads volumes.
According to your further explanations, I have to say ... this makes it a bit difficult to measure particle No. / volume. However, if you know any sort of quantity (e.g. weight) of the erythrocyte you initially used, I can propose yet another (new) equation. BTW, are you / your team interested to publish the new equation?
Another recent example of fluorescence method: https://doi.org/10.1016/j.dyepig.2021.109287
Still, an idea on mass balance of raw materials leading to vesicle formation may help.
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