I am trying to determine if there are still intact cells within dried, archived fish scales so that I can extract DNA. Will I be able to use an H&E stain to determine this and if so, how would I approach this? Thank you
Dear Loraine ,
I should say that for extracting DNA you can work on your samples whenever you want even with dried ones, but for H&E procedure it can be different as you must use fresh tissues for consideration of H&E protocol correctly.
For scales because of their died nature you need to make sure.
Cheers,
Milad
H & E always require fresh samples, or moribund!! even frozen and perish samples are not working in Haematoxylin adn Eosin stains.
Thank you for your answers. Could you recommend an alternative stain that might work?
Loraine Shuttleworth , since fish scales are bone like structures, therefore after bone digestion protcol you can give H&E staining a try.
Good luck
H&E won't work for this, staining isn't an option for this, u could try other measures
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