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Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Question about water vapor uptake of metal-organic frameworks?

I'm working on the atmospheric water harvesting, and i synthesized MOF by adding some materials into precursor and changing the conditions. (i followed Hydrothermal method) Actually, if MOF is...

24 July 2024 1,515 0 View

Is this a scam?

UK's Well-online Science Press sent an invitation to be a co-author and offers a payment of $30 per review.

22 July 2024 1,695 8 View

When you express a protein, why do we express not only the domain we want, but also the protein around it?

I want to express STK4, and I've searched the paper for reference. When I check the protein kinase domain sequence for that kinase on Uniprot, it's 30-281, but the paper expresses the protein...

20 July 2024 4,951 1 View

The reason for breeding Loxp mice with FLPe mice?

I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...

16 July 2024 7,475 1 View

Why is serum-free media used when making cancer cell conditioned media?

I am preparing cancer cell conditioned media to study the effects of substances secreted by cancer cells on other cells. In some papers, conditioned media is prepared using FBS-containing media...

11 July 2024 7,063 1 View

Stable cell line generation: GOI is detected by qPCR but protein is not expressed?

Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...

11 July 2024 4,812 5 View

NH3/NF3/H2 Remote plasma Oxi/nit selective etching mechanism help ??

Hello, I am an Etch Engineer. I am performing etching with high T_ox/Nit selectivity using remote plasma. Etching plasma is formed using only NH3/NF3/H2. 1. I wonder if it would be advantageous...

11 July 2024 8,115 2 View

Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

Is it appropriate to apply the COSMIN guidelines for evaluating the quality of tools used to assess acute postoperative pain?

Hi, I'm a graduate student, college of nursing, chungnam national university in Daejeon. The topic of my thesis is "Quality Assessment of Tools for Evaluating Acute Postoperative Pain." I have...

10 July 2024 3,048 1 View

In teaching with Evidence-Based Practices for students with ASD, what are some that can benefit from the use of AI?

I am a student in a master's program for Special Education and I am curious to see what Evidence-Based Practices that are used in teaching individuals with Autism Spectrum Disorder benefit from...

30 July 2024 5,611 1 View

Methods of teaching economics?

Answer should be based on institutional level

29 July 2024 1,506 4 View

Given currenct advances in the AI and Smart Computation, what are the prospects of having truly Humanoid Computing Devices in support of Education?

Today, many priorities for improvements to teaching and learning are unmet. Educators seek technology-enhanced approaches addressing these priorities that would be safe, effective, and scalable....

26 July 2024 8,544 4 View

What is the most appropriate pedagogical approach that suits or is best for teaching Elementary school classes?

Teaching is expected to bring changes in behavior and any pedagogical challenges faced at high schools is as a result of foundation which is the elementary school. So, which teaching strategy or...

21 July 2024 2,815 5 View

I would to know what are 10 different AI tools used in language research?

I would like to wirte an article on the use of AI tools in teaching speaking skills. What are the relevant AI tools that I can use to teach and guide students to use them? Thank you

13 July 2024 2,301 4 View

Does creative teaching influences positively in students' learning?

Creative teaching can lead to a wide variety of teaching styles.

12 July 2024 8,627 4 View

How well are first year teachers prepared to teach Early Literacy skills?

research on teacher preparation programs and readiness to teach early literacy skills

10 July 2024 2,177 5 View

How can technology empower EFL teaching?

Scholars from multiple disciplines stressed the importance of AI Technology in ESL teaching from a theoretical perspective. Are there teaching designs that combine theoretical discussions and...

01 July 2024 999 7 View

MGA Analysis for more than two groups ?

I did MICOM and obtained partial invariance for both my demographics: teaching experience. I split the teaching experience into three groups to do Multigroup analysis However, I am not able to...

28 June 2024 3,721 4 View

Is there someone good in generating special quasi random structure ??

I want someone to teach whole ATAT tool kit Especially mcsqs and sqs2tdb I will pay for the classes

27 June 2024 8,496 0 View