04 April 2016 3 6K Report

Need to do immunofluorescent staining on human tissue.

More Cheng-Ying Ho's questions See All
What is the best kit/method of RNA extraction for mouse DRG for RNA-seq?

I have tried Qiagen RNeasy Micro and Fibrous Tissue Mini. The yield is both low. I am wondering if there are better methods/techniques to increase the yield.

11 December 2019 6,769 3 View

How to keep DRG neuron cultures at the center of the plate?

After plating the neurons look okay but they all migrate and clump at the edge of the plate. How can I keep them at the center of the well? I am using the 6-well plate. I was told to place a drop...

07 August 2019 5,647 3 View

Any good BDNF antibody against human and mouse tissue for Western blot?

Since Santa Cruz discontinued their awesome rabbit antibodies a year ago, I have yet to find a replacement. Any recommendation on antibodies for Western blot? The antibody must recognize the...

31 December 2017 6,878 5 View

Best transfection method for primary keranitocyte cultures?

What is the best transfection method for RNAi or CRISPR/Cas9 on primary keratinocytes? What is the maximum efficiency achieved?

10 November 2017 5,998 5 View

Gene expression profiling (gene chip) vs. RNA-seq

Need to conduct gene expression studies using cell lines (treated with a drug vs. no treatment). Is one method better than the other? Cost is a consideration too. Also want to know how many...

07 August 2017 10,005 5 View

What is the best method to analyze clonal architecture of cancer based on whole-exome or targe panel sequencing data?

I am looking into PhyloWGS. Can someone like me without bioinformatics background use it with ease? 

01 February 2016 9,660 3 View

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