What is the best way to measure and compare the signal intensity in Sigma Fast DAB labelled pyramidal cells in paraffin embadded tissue?

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Esha Gauba

Hi Adam,

I usually quantify the DAB staining with ImageJ Software using Color Deconvolution plugin. After using the plugin for DAB staining, I use a good number of images (for eg. images with maximum signal and least background and some with very high background) to find the correct threshold where I have maximum signal and least background for all the images. I apply this threshold limit for each image and get the signal intensity.

I can provide further details if you would like to use ImageJ.

Best Regards,

Adam Legradi

Hi Esha

Thank you very much your answer. I would like to use ImageJ also. I have a lot of slides, and I would like to measure a huge amount of cells. How I can set treshold? did you measure integrated optical density or mean grey value? Please send me a detailed protocol if it is possible, or attach a publication. Thanks a lot :

adam

Adam,

You can try the following method for quantification-

https://www.researchgate.net/post/How_do_I_quantify_DAB_using_ImageJ

HI Esha

thanks your answer