Hi, I want to induce DNA damage using the UV light in the laminar flow hood?
I inquire is that possible and what is the required duration?
Thanks,
Hello Nehal Ibrahim Ghoneim
You may try the following protocol. But whether it can be done in the laminar hood. Not very sure.
1. Seed cancer cells in the required plate.
2. After 24 hours, remove the growth medium and expose the cells to UV light (3.18 ± 0.2 mW/cm2) to induce DNA damage.
3. The time factor is also important. So, you should try different UV light exposure times (say 1min, 2min, 3min, 4min and 5min) and evaluate the DNA damage with Comet assay. Choose the time point which does not induce cell death.
4. Simultaneously, you should also have cells unexposed to UV light and left without growth media for the same time points. So, you will have two groups of cells, UV-treated and UV-untreated.
5. Proceed with the two groups of cells to the next step as per your requirements.
You may want to refer to the article attached below for more information. It will be helpful.
https://www.journalofdairyscience.org/article/S0022-0302(12)00587-5/pdf
Good Luck!
- Badges
- Science method