Dear Sandy Cole ,

You could probably start from collecting information on anti-CD25 (IL-2Rα).

For example for humans:

https://www.biolegend.com/en-us/products/pe-anti-human-cd25-antibody-616

And for mice:

https://www.biolegend.com/en-us/products/pe-anti-mouse-cd25-antibody-424

Regards,

Yes, after I sent this question, I looked up IL2R in BD's catalog and it looks like it's synonymous with CD25 so pretty straight-forward. Thank!

These research group did.

you may read their method.

Original Paper

Interleukin-2 expression in human carcinoma cell lines and its role in cell cycle progression

Torsten E Reichert, Shigeki Nagashima, […]Theresa L Whiteside

Oncogene volume 19, pages514–525(2000)