Using two clones against human FoxP3 in combination is supposed to increase sensitivity for detection of these cells ( Article Association of Two Clones Allows for Optimal Detection of Human FOXP3
) .Not all combinations of clones are compatible as far as I recall, and some may stain activated T-cells non-specifically.
I would like to know if clones 236A plus 259D are a suitable combination to achieve this, or if you could recommend any other combination.
I am looking for a clone available conjugated to APC or AF647 for an existing panel. This is for use with human PBMCs from an autoimmune/inflammatory environment (i.e. with potential for activated T-effector cells being present).
This may be of interest to you:
Article The importance of Foxp3 antibody and fixation/permeabilizati...
Thank you- yes, this was very useful. Sadly some of the clones used in this are no longer produced in the fluorochrome I am fitting onto my existing panel. But great for future panel design.
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