I need some information whether it is possible to extend the logarithmic scale (x axis, MFI level) in flow cytometry histograms? In my case the maximal value is 104, which is to low for some samples. I can not manipulate voltages due to control and isotype control start to be invisible.
Thanks in advance for any suggestions.
Magda
Hello Magdalena,
I am not sure if your problem is in a data analysis program or with the analyzer itself. If the data is recorded in an analyzer then you cannot manipulate it afterwards, meaning the data doesn't change. It seems that the range of your analyzer is 10^4 which in most cases is perfectly fine. You are also saying that your control (negative population?) is invisible if you lower the voltage of that detector? You mean the population will go of the axis? I need more information from you to give a more specific answer. The log scale is a fixed range in the analyzer, usually with a analyzing program you can extend this fixed range, but it wouldn't look prettier. You can lower the antibody concentration?
Best regards,
Johan
Hello Johan,
thank you for response and suggestion. I try to use the lower concentration of antibodies.
Best regards,
Magda
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