How much the nanoparticles reach the inflammatory tissue?

Dear colleagues, I want to know the average analysis data of EPR effect on inflammatory tissue (acute lung inflammation/injury, ischemia/reperfusion, rheumatoid arthritis, and sepsis..) In...

22 December 2020 3,147 2 View

How to measure the bonding strength of transfered graphene on PDMS substrate?

Chemical vapor deposition (CVD) was used to grow graphene on copper (Cu ) substrate, then the single layer graphene was transfered onto a PDMS substrate. I want to measure the bonding strength of...

17 October 2020 3,567 3 View

Why the mice muscle fiber contain holes in IHC detection?

When I performed Hoechst staining or HE staining in muscle fiber (Gastro muscle), it could be noticed very frequently that some fiber contained holes in the middle. Cyrosection was used in that...

15 July 2020 6,910 4 View

How to define the particle agglomeration in composite materials or foundry alloys and measure its size distribution quantitatively?

28 June 2020 7,614 3 View

What form of Mg will be selected during the purification of prokaryotic expression protein?

Hello everyone Recently I wanted to purify several proteins by prokaryotic expression, I read some related literature and found that Mg is needed in the protein purification buffer. I am a little...

02 May 2020 931 3 View

Free acid and its salt, can HPLC-PDA-MS tell its difference?

I have a free acid R-COOH and its salt R-COOM. Both are insoluble in water but soluble in methanol. When their methanol solutions are injected into a HPLC-PDA-MS system, are they going to have the...

06 March 2020 4,952 4 View

Sodium chloride solution saturated vapor pressure at sub zero temperature?

Can anyone suggest where to find the data or any equations related to the salt solution vapor pressure at sub-zero temperature? Many thanks!

24 December 2019 7,741 2 View

[Systematic Reviews] What to do if publication bias exist?

When doing the systematic reviews, if you I confirmed a publication bias during meta-analysis, my questions is what to do next? I though to add discussion about the publication bias, but I want...

17 November 2019 5,634 4 View

How does acridine orange works to recognize apoptosis, autophagy or lysosomal stability?

I used acridine orange staining to detect autophagy. First, the cells were grown in RPMI 10%FBS in confocal dishes, treated with compound with or without with wortmannin (0.5uM). Wortmannin was...

08 August 2019 9,319 5 View

If two shRNA linked together, it works or not?

03 August 2019 299 3 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

Are E.coli strains M15 and pET28a vector systems compatible? Can I over-express my protein which is of plant origin. Previously I was using BL21 DE3 strains. Was confused to use M15 strains for...

10 February 2021 2,564 3 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What are the benefits exactly of synthetic intron (IVS) and internal ribosome entry site (IRES) on a plasmid?

Hello everybody! so I have a plasmid which has a synthetic intron (IVS) & an internal ribosome entry site (IRES), and I'm thinking to remove them as I'm cloning due to some matters related to...

26 January 2021 7,645 2 View

Can A. thaliana splice algae genes?

24 January 2021 329 2 View

Is MOPS buffer needed for in vitro horizontal gene transfer in thr coculture of two different pathogenic bacterial species?

Actually i am working on possibilities of horizontal gene transfer of resistant gene in broth coculture of two different types of bacteria, and the protocol i follow involves the use of MOPS...

19 January 2021 4,566 2 View

How can I insert antibody sequences into yeast cell to achieve stable and large quantity production?

I have the sequence of the light chain and heavy chain of a certain antibody. I would like to know how to express this antibody in yeast cells to achieve stable and large quantity production. It...

04 January 2021 9,669 2 View

How to prediction non-visible parts of microscopic pictures?

Dear all, I like to ask if someone has experience with evaluating pictures towards the prediction of non-visible parts. I have confocal-laser-scanning microscopic pictures of yeast cells and want...

28 December 2020 4,309 11 View