What is the difference between Bacterial DNA and eukaryotic Mitochondrial DNA?

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Does YeGFP expresses in mammalin cell lines like HEK?

I have cloned YeGFP in a mammalian expression vector, but I don't see any GFP expressionin HEK cells. Is YeGFP is supposed to fluoresce in mammalian cells?

09 October 2018 9,033 1 View

Can Laminin be used twice for mice cortical neuronal culture?

I used laminin at a concentration of 10ug/ml for growing mice cortical neurons. Can I store it in -20 and re-use it?

09 October 2018 9,712 1 View

Is there any protocol for myelin coating?

I am extracting myelin fro rat brain following Norton and Poduslo, 1973 method, but I don't know how to use or process the myelin after that. Also, I couldn't find any protocol for plating myelin....

10 November 2017 855 3 View

What concentration of Laminin is ideal for neuronal culture?

What concentration of Laminin is ideal for neuronal culture? And should I dissolve it in water or PBS?

08 September 2016 7,550 11 View

Is there any different way of staining for electroporated neurons?

I am doing electropration in my neurons with some construct that has been tagged with GFP. Now I want to stain the neurons for Tau protein in red. I am getting very very faint or no staining at...

04 May 2016 7,243 11 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View