Can anyone specify the reasons behind the different microorganism inhibitory patterns between aqueous and methanolic plant extract (disk diffusion) ?

I have prepared an aqueous extract and a methanolic extract of a plant. Using the disk diffusion method, the microorganism inhibitory pattern I am intended to observe. In several literature...

20 December 2020 9,830 11 View

RS dataset of ice cover of Arctic Antarctic?

I am looking for datasets of ice cover of Arctic Antarctic. I want only India related dataset. If you have any source of dataset please share with me. It will be very helpful for my...

13 October 2020 7,137 1 View

Can anyone suggest a Digital Bandpass filter in MATLAB?

I want to filter out some unwanted information from a 2D image data for image processing. Can anyone suggest a Digital Bandpass filter in MATLAB regarding this purpose? Best Regards Anik Ghosh

31 July 2020 1,434 10 View

Is there any simulator for Hyperledger Fabric?

I was looking for a simulator to simulate a proof of concept in hyperledger fabric by changing the number of the orderer, endorser, organisation, and also wanted to apply consensus in the network....

06 April 2020 5,943 6 View

How can we get the individual and time effects in a two way fixed effect model using R?

Currently when i run the code i get only the coefficients for independent variables. I dont get the specific intercepts for each entity and each time period. I need separate equations for each...

31 March 2020 4,540 4 View

Can I use RII and SII for women empowerment index?

Hi, I have constructed a women empowerment index (one of the independent variables) using PCA with 3 quintiles: low, medium, and high. Now, if my dependent variable is a public health problem...

29 March 2020 7,500 3 View

How to investigate (multi)collinearity for categorical variables?

Hi, I was wondering about finding possible multicollinearity relationship among the categorical independent variables. I thought it would be VIF. But, VIF didn't provide me good result (all were...

22 November 2019 5,330 4 View

Co-authorship in a research paper and its effect on impact factor or the overall weightage of the research paper?

Does an increase in the number of co-authors have a negative effect on the impact of the first author or the corresponding author? Is the total impact factor of the paper equally distributed among...

20 November 2019 1,767 7 View

What is opposite of network Throughput?

As we know that, throughput is amount of transferred data for a specific time. I want to fix data size and make time variable. The definition will be "The amount of time required to transfer a...

10 December 2018 9,326 10 View

Which software to use for trimming sanger sequenced data, making contigs and mapping to reference gene?

Hi, I have an amplicon of 1.6kb and I have sequenced it using forward and reverse primer. Now, I have to trim both the sequences and assemble those into one contiguous sequence. My goal is to...

03 September 2018 2,923 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

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17 February 2021 4,217 3 View