One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in... | Contact experts in Eukaryota to get answers
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Questions related to Eukaryota
Please, does anyone know the ratio or percentage of pyrimidine bases found in bacteria in comparison to yeasts or other eukaryotes? Thanks so much!!
08 August 2019 8,625 5 View
Is there any assay that can measure eukaryotic protein translation from mitochondia apart from radiolabel S35 methods? Anyone has experience with superfolder GFP used in yeast?
06 June 2019 9,209 1 View
I want to express a eukaryotic protein using E.coli. I assume that codon optimization may be helpful. I tried both GeneScript and ThermoFisher GeneArt. The two company generated different...
05 May 2019 8,608 4 View
Hi, I am conducting a RNA-seq project on micro-algae cultures. As my samples both contain algae and bacteria, I would need to remove both bacterial and eukaryotic rRNA. Though, Illumina does not...
05 May 2019 6,837 7 View
I have metagenomes samples and I would like to align my reads to all the genetic/genomic information about eukaryotes. But first I would have to build a database comprising this information. Any...
04 April 2019 6,631 3 View
In eukaryotic organisms there are transcription factor families in which basically the DNA binding domain is common but the rest of the sequence varies widely. What is known about the origin(s)...
04 April 2019 4,048 3 View
Is there any database or online tool to find the TSS in promoter sequence of Eukaryotes. Thanks
04 April 2019 5,305 3 View
I need to obtain a long stretch (specific amount) of the sequence from the upstream (Upstream of the start codon) of a particular gene.
03 March 2019 2,999 5 View
Hi, I am trying to detect Eukaryotic RNA contamination in my bacterial RNA samples. I plan to sequence the bacterial RNA to get a transcriptomic profile. I am aware that qPCR can show if there is...
03 March 2019 5,557 2 View
I am working on an extracellular matrix secretory putative protein(Eukaryotic). Through Phosphoproteomics studies, I got to know that phosphorylation sites are available in this protein. The...
17 January 2019 8,599 10 View
01 January 2019 8,383 11 View
Hi there, Is there any software tool or database to identify the entire 5' and 3' UTR regions of bacterial gene? I am aware that eukaryotic genes are clearly annotated in Ensembl and Genbank with...
01 January 2019 2,900 3 View
Does anyone have experience if there exists quantitative PCR 16S primers for bacteria, not eukaryotic mitochondrial DNA?
01 January 2019 10,085 0 View
for example in this electrophoresis we have the cell, the positive, the negative, a control and another cell. why the first cell has only the band of prokaryotes (between 1500 and 1200 bp)?
01 January 2019 7,838 0 View
Storeatula major is an eukaryotic marine micro algal strain. For a research project, we are trying to find the suitable cryopreservation method of this strain.
16 December 2018 4,402 3 View
PCR amplification of 16S/18S rRNA gene using universal primers
12 December 2018 6,252 2 View
I cloned my eukaryotic gene and sequenced it, now I have a problem in protein expression. The target protein still does not appear. Can anyone help me? I clone eucaryotic gene into e.coli using...
12 December 2018 6,983 16 View
12 December 2018 9,798 4 View
I have read many papers working on arabidopsis and Heterodera schachtii, in which the cysts, eggs and J2 juveniles were washed by chlorhexidine, chlorhexidine, sodium azide, mercuric chloride,...
12 December 2018 2,576 4 View
07 December 2018 4,372 14 View
05 December 2018 7,856 3 View
Fungus
11 November 2018 1,056 11 View
Dear all, I have started a project on soil biodiversity recently and my target is mtDNA. Most of the articles I read is focus on microbial diversity, which also a part of my research. But I want...
11 November 2018 3,132 0 View
Eukaryotic
11 November 2018 3,196 2 View