Does the usage of shuttle vectors gives a better result in eukaryotes or prokaryotes?

More Chintamaneni Shanmukh Chowdary's questions See All

Is their any standardized protocol Construction of Corynebacterium glutamicum vectors for producing glutamic acid?

Corynebacterium glutamicum is an important industrial strain used for the production of amino acids and vitamins. So I want to know is there any standardized protocol for the Construction of...

13 August 2020 1,679 1 View

Net plot yield converted into hectare yield or yield parameters of selected plants converted into one-hectare population yield? which one is accurate?

Every time this is an issue arising while calculating the yield. Two different kinds of results are coming with two different approaches

19 June 2017 1,787 5 View

How can i calculate pull force of a linear actuator(solenoid) with a NdFeB core?

I came across a formula F=[[sq(n*i)]*4*pi*10^(-7)*A]/2*sq(g). But the terms A and g aren't properly explained and also, the magnetic field of NdFeB core is completely neglected here.

15 March 2016 4,551 4 View

How can I prevent the plasmid vector from closing itself and becoming circular again without our gene of interest during ligation activity?

A circular plasmid vector has been cut with a specific restriction enzyme to incorporate our gene of interest into it. How can we prevent that vector which is now linear from binding to itself and...

19 August 2015 3,603 5 View

Is there any new development in measuring Intra-Industry Trade other than Grubel Lloyd Index?

Intra-Industry Trade: Exporting and Importing the same product by a country. Grubel Lloyd Index is the general measure of IIT

07 April 2015 2,484 3 View

What is the concentration of ethylene needed to treat unripe mangos (Alphanso) to ripen them within 2 days?

In normal conditions or traditional ripening practices (keeping the fruit in a covered box with paddy stow) these fruits require 7-8 days to become ready to eat. We want them ready to eat in 2...

16 April 2013 5,586 7 View

Production of pro-biotic alcoholic beverage, like toddy which helps to humans...and energy source also replacing with pulses

Fermentation

27 December 2011 8,179 3 View

hmmm letz cee how dis works

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18 October 2010 5,200 2 View

DEM LIGGGHTS- Compression of sand particles in soil plate

I have a compressed/compacted density & volume fraction of sand particles in soil plate. It was prepared by inserting particles and rammed it. Can I directly use compacted data in LIGGGHTS...

01 January 1970 1,013 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Milad Mollaali

Hello. This may help you.

http://www.freepatentsonline.com/6627436.html

Abhijeet Singh

What is the your definition of usage and better result. What is your actual aim?

Nothing is good, better or best if you don't have any aim or reason.

Ali Javadmanesh

It depends on your vector structure as well as the insert, please read the user instructions.