I have some sporocarps of gilled mushrooms frozen at -20°C and I want to regenerate the mycelia from them. can it be used as inoculum?
if YES,
Please suggest me available methods to do so.
As you might guess, the answer is "it depends". First, what was the condition of the sporocarp when it was frozen? Was it fresh with no decomposition. Second was it a clean specimen without soil and bacterial contamination? Third, is the temperature of the freezer uniformly -20? Any fluctuation could result in breakage and rupture of the mycelium. Fourth, did you use a cryoprotectant to protect the sporocarp from freezer damage and damage to the cells? Fifth, how long has the sporocarp been in the freezer - more time in the freezer, the les chance you have of getting viable mycelium out.
If all these points are favorable, I think you have a good chance of getting a mycelial culture from your sample. I would recommend washing a sample of the tissue with warm (30 degrees sterile media, then place the sample in fresh sterile, warm media in a water bath, again warm, not hot, to thaw the tissue - not too fast, not too slow. When the tissue sample is thawed, gently macerate the sample and plate out on solid media. I would make multiple sub-plates with the macerated tissue to maximize your chances of getting an uncontaminated mycelial culture. Best of luck.
Agree with Clifford Keil above - the only way you will find out is to try it.
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