We are applying ultrasound for live cells grown in multi-well dishes. There is no a noticeable effect on cell viability. How to show that there is an effect of ultrasound at all in the cell medium?
Honestly answered to help for the weekend (;.))
Dear Vilma,
unfortunately I am not more active in such research but have read at least some scientific-biological papers on ultrasound effects on cell(s, cellular milieu, in terms of lght&electron microscopy) the last 15-20 years (and have had a research project on Effects of ultrasonic aspirator on endothelial cells in human vasculature in the 1980ies).
You stated: "no noticeable effect on cell viability", so it would be interesting what / which measures and methodology you used up to now to come to a / that particular conclusion.
I would like to invite you to open GOOGLE.com for search, insert
| Ultrasound effects AND cell milieu experiment method |
as the search phrase and see what is contained in the www-sources [ out of the first 20 results: e. g.: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437622/ = 2017 = https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-017-0364-3 , cf. Methods section! ].
Do the the same with Google Scholar [e.g.: https://scholar.google.at/scholar?hl=de&q=Ultrasound+effects+AND+cell+milieu+experiment+method&btnG=&lr= ] and - last but not least:
ResearchGate archives (searching "PUBLICATIONS" for e. g.: | Ultrasound effect cell experiment method | .
Naturally you can change and modify your search string using AND in between the single keywords or search only for one or two specific keywords which you put into parenthesis like
"XXX space space YYY".
Searching RG-Archives in "QUESTIONS" will not lead to conclusive or specific results you could use for a first and rapid orientation (Hoping you know how to search RG-Archives?).
Apologies for not having at hand for now a 100% conclusive and satisfying answer to your specific but more generally described "problem"...[histological, immunohistochemical/immunocytochemical - light-microscopical / ultrastructural approach?] knowing the specific literature is the best base to be successful in your methodological choice.
Best of luck and wishes!
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