AAS dried plant samples digestion protocols?

I am looking for alternative sample digestion protocols for dried herbs to be analyzed with AAS for presence of Pb, As and Cd. So far I have attempted acid digestion over hot plate (HNO3 and aqua...

28 December 2020 7,765 5 View

How to draw ROC curve for the correlated parameters?

Dear Fellow Researchers, We want to access the prediction accuracy for various parameters. We were able to draw ROC curves for each of the parameter separately using SPSS. Now, we want to see a...

21 October 2020 4,994 3 View

How can I analyze qPCR data without control sample/calibrator?

I would like to compare expression of my gene of interest (GOI) in non-treated leaves, stems, roots of my plant. In that case I don't have any control sample here and it's not possible to have...

09 August 2020 3,998 3 View

Is it possible that dicot plant could rearrange or excise from his genome transfected DNA fragment?

I have transfected Salvia milthiorrhiza plant using Agrobacterium tumefaciens cells carrying binary plasmid. At the level of Agrobacterium I confirmed presence of my binary vector by obtaining...

26 June 2020 8,763 15 View

Where can I find suppliers of rare dyes?

I have trouble finding certain dyes for my research (mostly acid dyes). I have approached producers of textile dyes from various countries, but the C.I. I am looking for are extremely hard to...

26 March 2020 5,648 6 View

MatLab -curve fitting?

Dear All, Could anyone help me please? I want to estimate the contribution of each curve (EA) to best fit the M curve (the bold one). Best regards Malgorzata

24 March 2020 8,316 5 View

Training and the environment?

Hi, I am looking for current research on training in high polluted air conditions and environment (running, cycling, rowing ...) Effects in plus and in minus (short- and long term). Can anyone...

02 January 2020 3,916 2 View

Any anti-HLA-B38/B39-specific antibodies?

Dear Fellow Researcher, I am working on HLA-B*38 and HLA-B*39 molecules and looking for the antibodies that can recognize these two MHC class I alleles. I do have W6/32 and HC10 antibodies that...

02 December 2019 6,693 1 View

How to evaluate the quality of the studies included in the systematic review?

Dear Fellow Researchers, We are writing a systematic review based on the various types of the studies: clinical trials, clinical case series and the animal studies. What type of study assessment...

17 November 2019 3,131 3 View

Which kit would you recommend for isolation of RNA from dicotyledonous plants (leaves, stems and roots)? Which vendor would you recommend generally?

My plant material is rich in secondary metabolites. With the isolated RNA I would like to examine expression of interested genes in Real Time PCR.

12 November 2019 5,338 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

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28 February 2021 9,936 2 View

Which is better medicine or food in obesity?

Preference in terms of the scientific aspect

28 February 2021 796 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View