I am using the sephadex as stationery phase and didtil water as moblie phasem in column chromatography. So my question is how to isolate the fractions obtained via column chromatography for the solutions of gamma irradiated sodium alginate.
I am having a hard time trying to understand your question. Anyway just add the sample to the column. The important things that you need to answer are:
1. Is your sephadex type appropriate for your desired separation?