We have transgenic E. coli and we need to isolated and purified the specific protein. We used membrane filtres, however it didn't work as we expected. We don't want to use chromatography for purification, first. I just looking for some other alternatives.
Thank you for response.
Is the protein tagged? If so you could use magnetic beads to run batch purification procedures.
I am not sure if I understand your question correctly. You objective is to isolate & purify the target protein. Filter/Chromatography is not what you want, however usually biosensor is good at isolation rather than purification. I do see some "catch and release" system for biomarker released from live cells.
For example:
https://www.nature.com/articles/nnano.2016.284;
https://www.nature.com/articles/nchem.2203
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