Why some results are quite different between the presence and absence of serum?

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Robert Kiss

Serum contains myriads of growth factors and ions that interfer and/or directly act on cell proliferation, migration and death.

You can even obtain very distinct data whether you use bovine versus human serum (see the attached article).

Best regards

Robert

Yuxuan ma

Hi, Robert.

Thank you for your reply. After reading the article, I realize the importance of purchasing regents from reliable sources and avoiding controversial results.

Yes indeed Yuxuan!

Akash Sabarwal

Serum contains all the essential growth factors. Removal of serum causes Gap 1 phase arrest of the cell cycle. Thus if you do your experiment with or without serum you will get difference in your results.