Does anybody have experience with miRNA mimics for cell transfection?

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Jiten Verma

Hi,

miRNA mimics transfection is a very simple technique and you can transfect them similar to siRNAs. Use RNAi max reagent from thermo scientific....

all the best...

Richard von Furstenberg

Hi Jiten,

So you have the miRNA mimics made as regular oligos and then get them into the cell using the RNAi max?

Thanks

Rich

Hi Richard,

I have used miRNA mimics from Sigma.....and they worked perfectly for me....

u can check the overexpression of your miRNA by using real time PCR using miRNA specific taqman probe...

Jiten...