[flow cytometry] txt or cvs file to FCS file?

Hello, I'm looking at converting txt or excel file into FCS file (running windows 10). Anyone could point me to a tool or software? many thanks, Vincent

11 December 2016 1,418 3 View

What is fluorochrome photostability/photodegradation?

Hello, When people are mentioning that for ex, Alexa fluorochromes are more photostable, I understand that they refering to their better photostability (reduced photobleaching) after a short (few...

09 October 2016 5,845 1 View

Mono/macro in human PBMC?

Hello, Can anyone share microscopy images showing monocytes/macrophages in human PBMC? or point me towards articles/resources showing them? many thanks.

08 September 2016 8,630 3 View

How to get T cell proliferation within human PBMC?

08 September 2016 5,258 11 View

How can I improve the sensitivity and dynamic range for my competitive ELISA?

At the moment I'm trying to optimize a competitive Vitamin B12 ELISA. It worked very well for a range between 200 pg-1ng, but unfortunately I need a range of 45pg-1ng. Can you give me any advices...

06 July 2016 9,256 3 View

How long does an antibody take to interact with its target antigen ?

How long does an antibody take to interact with its target antigen ? When performing an ELISA, why are we "traditionally" incubating antibodies etc for 1 hour or more?

06 July 2016 6,285 7 View

Relative proportions of sub-populations in mouse spleen, LNs, blood?

Hello, What are the relative proportions of the sub-populations in mouse spleen, LNs, Blood? any article or link to recommend? Many thanks.

09 October 2015 7,059 2 View

Would CFSE/CD4 staining be fine in an assay where all splenocytes are restimulated?

Hello, I would like to setup a proliferative assay, I don't have access to irradiation. So instead of using irradiated splenocytes for an thymidine incorporation assay, would CFSE/CD4 staining be...

08 September 2015 5,952 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View