I am observing black dots or debris kind of stuff into culture dishes, fresh media, fresh cell revived but the problem persists. This does not make the media turbid but. This debris is less on the day of splitting, however, it increases with time.
Check for mycoplasma infection, bacterial or yeast. Are the cells dying?
Do a quick negative stain TEM on the material.
degradation of cells occur in all cultures...deaths or infected medium of course, to be revealed with TEM is best
Hi,
I would be careful with splitting, you might exposure the cells to harsh condition such as leave them too long in trypsin or using high concentration of trypsin and so on. so I would be more careful with splitting technique
Massar Alasamraae we are using same concentration of trypsin since many years and exposure time is also kept same, however, from past 3 months all of my lab mates are suffering with the same problem.
Hi!
I had same problem and it was bacterial infection. I doubt you'll be able to get rid of them. At least I was not able to, despite great efforts.
- Badges
- Science topic
- Similar topics
- Medicine
- Oncology
- Cancer Research
- Cancer Biology