Does anyone know the reason of the formation of protein aggregates after subjecting a protein isolate to a sonication treatment?

Jesús Eduardo Pizano Galvez @Jesus-Pizano-Galvez

30 July 2024 1 4K Report

After solubilizing a protein isolate in water at pH 7, it was subjected at a sonication treatment in ultrasound bath at 40 kHz durign 10 minutes. After that precipitation was observed.

Sergio J. Calva-Estrada

¿Pudiste encontrar la respuesta a tu duda colega?

Badges
Science method

More Jesús Eduardo Pizano Galvez's questions See All

What are novel cryptographic protocols that enhance email security?

what are novel cryptographic protocols that enhance email security?

13 June 2024 7,090 2 View

Hello, very good day everyone. I wanted to ask how many rpm to obtain the separation of a drug sample in wastewater with activated sludge?

I need to separate my drug from a sample with activated sludge and residual water

17 April 2024 9,601 2 View

How to get B.inp GGA for generate vdW pseudopotential of atom in SIESTA?

On the page I currently cannot download it, or if you can guide me to generate it.

21 February 2024 4,374 0 View

¿What is the affinity of serotonin (endogenous) for the 5-HT1 and 5HT2 receptors?

I need to know if there are measurements or studies on the affinity of serotonin (endogenous) for the 5-HT1 and 5-HT2 receptors

03 February 2024 5,878 3 View

Why MiFish Primers amplify when they're alone but not when they have an overhang sequence (Illumina adapter + tag)?

Good day everyone. I'm working with eDNA Metabarcoding for freshwater fishes in Brazil, I ran tests amplifying positive controls and samples with the primer MiFish-U (Miya et al 2015) alone (no...

11 January 2024 5,109 2 View

What are the specific challenges and methodological best practices when conducting a meta-analysis in studies related to cancer?

How can these methodological considerations impact the reliability of drawn conclusions and their relevance to cancer research and treatment? I am currently exploring the realm of meta-analyses...

20 November 2023 6,056 2 View

Do someone know the heat transfer coefficient of ice?

I just need the Hc

21 October 2023 5,162 3 View

ERA5's sensible heat radiation in W/m^2 is the same as mean sensible heat radiation?

If I convert J/m2 to W/m2 the reanalysis variable surface latent heat flux, will there be any difference with the variable mean surface latent heat flux? Or is it basically the same when you do...

15 September 2023 8,999 0 View

Predators of Thrips parvispinus?

Good evening: Does anyone know what are the natural predators of Thrips parvispinus? Thanks in advance Jesus

26 July 2023 4,659 2 View

Does refactoring mean that you modify the entire design iteratively?

It is posible refactoring and no modify they entire design iteratively?

19 June 2023 6,836 3 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,920 11 View

Is it fare for editors of reputed Journals to put on hold manuscripts more than six months without review updates, Are they harassing researchers?

It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...

24 July 2024 8,596 2 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

15 July 2024 2,414 1 View

Has anyone tried freeze flow samples in -80C freezer or liquid nitrogen before processing?

Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...

15 July 2024 9,975 1 View