What is the meaning of this type of melting curves in qPCR?

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Alessandro Paparella

No right concentration in probe and primers

Amy Klocko

My best guess would be lack of amplification. Have you tested your primers with end-point PCR and if so do they give a product?

If you re-try the QPCR and see the same melt curve, you can run your QPCR products on an agarose gel to see if you get bands or not and their size.

Hope this helps!

Camilla Mandorino

Thank you very much! That is exactly what I did, I ran on agarose gel those qPCR products comparing them with a good amplification product and I could not see anything, so I had no amplification at all!