Hello. Everyone.
I'm struggling to find some effective methods for detecting mutations in a plant genome or gene level, using mutated plant lines, without WGS or NGS.
Is there any recommended approach?
I appreciate your time.
You can use commercial kit for detection. Its available on thermo or life technologies.
Its more easy and reliable.
You can use commercial kit for detection. Its available on thermo or life technologies.
Its more easy and reliable.
In the RFLP/PCR (RFLP, restriction fragment length polymorphism) approach of 'genotypic' mutation analysis base pair changes, small deletions and insertions are measured which are located in a restriction enzyme recognition sequence and render this site resistant to cleavage by the corresponding endonuclease.
Article Genotypic mutations analysis by RFLP/PCR
SDS-pcr DNA
ويختلف حسب نوع الصفة المراد تحديد الطفرات لها
You can use RFLP methods for Mutation Analysis.
Analysis of mutation/rearrangement frequencies and methylation patterns at a given DNA locus using restriction fragment length polymorphism
https://pubmed.ncbi.nlm.nih.gov/20204868/
After multiple sequence alignment of your gene of interesr of plant, you can predict difference of protein, then you can further analyze with SNP or SSR markers to get variation
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