there are tiny movable dots and rods like strucrture and there are filled in my cell plate. i have no alternative but to eradicate them from the cell as I have no other stocks left. so How can I be able to remove them?
I want to know proper process for the concentration of gasoline present in soil and after removal of gasoline, how much quantity left in the soil. So, can anyone please tell me the detailed...
05 February 2024 9,718 1 View
I want to know the composition of high volatile compound present in soil. So, which technique will give me the right information of all organic elements present in that high volatile compound? As,...
30 October 2023 9,035 1 View
Currently, We are using free floating section in 12-well plates and small baskets to transfer tissues from one well to another. I have a few specific queries: Could you please provide insights on...
03 August 2023 5,523 2 View
I am currently working on IHC for Per1 protein expression in the suprachiasmatic nucleus of mouse brain coronal sections 40um thickness. The ABC kit that I have, has the expiry date of June 2022....
29 June 2023 3,270 5 View
I am working on Immunohistochemistry for Per1 protein expression on mouse brain coronal sections (40microns thickness, free floating). In the protocol that I'm following, it says that the DAB...
29 June 2023 2,647 4 View
I am working on Immunohistochemistry on mouse brain and have sectioned 40 microns thick sections as per the protocol. I will be using 12-well plates. How many sections are advisable to be kept per...
12 June 2023 9,602 6 View
I want to stain some of my mouse brain sections with Cresyl Violet. The mouse brains (not perfused, no PFA/Formalin used) were fresh frozen in OCT and cut in the cryostat at 15μm thickness. The...
16 May 2023 7,657 4 View
Characterizing a GPCR-ligand interaction is critical to understanding the biology of the receptor. As GDP/GTP exchange is one of the earliest events that follows ligand binding, monitoring GTP...
08 May 2023 2,185 2 View
Can anyone please explain about purpose or role of FTIR Characterization in bimetallic nanoparticles? As we can't identify any functional groups though. We can identify 2-3 oxide peaks, O-H group...
20 April 2023 4,902 3 View
In catalase test, we add H2O2 to test the presence of catalase enzyme.If the bubbles do not appear, It confirms that it is a lactic acid bacteria. But to test antimicrobial activity by...
19 February 2023 549 4 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
What can be a hypothesis related to the Stock out Cost or Shortage Cost?
29 July 2024 9,259 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View