I am currently working with the Panc1 cell line and have observed some black dots or thread-like structures in my culture. These structures do not seem to interfere with the growth of my cells. After washing the cells with PBS, the black dots reduce but reappear within a day.
I would like to ask:
Hello Vandana,
1. These black dots do indicate contamination, and they are not a byproduct of cell metabolism. They are attached to the plate surface and that is why, washing reduces their number but can't get them rid of completely. Did you check them under high magnification to see if they are motile?.
I do recommend to discard these cells and thaw the back up vial, if you have any. If not, wash with PBS harshly once every 1 hr for one day and do a single cell dilution.
2. The cells definitely look stressed.
Good luck.
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