Why albumin coagulate in anti-inflammatory assay ?

More Mohammed Adil Selka's questions See All

Is skin yellowness an numerical or ordinal variable?

I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...

11 August 2024 4,793 1 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

How can I get old refernce in geomorphological mapping?

how can I read Waters, R. S. 1958. Morphological mapping. Geography 43 :10-17 from internet? note: not in google or resaearch gate

26 July 2024 7,813 3 View

How can the rubber fibres produced by the electrospinning device be removed from the fibre collect(aluminium foil) ?

How can the rubber fibres produced by the electrospinning device be removed from the fibre collector (aluminium foil) without affecting the orientation of the rubber fibres for use in reinforcing...

26 July 2024 8,281 0 View

My question concerns MTT cell viability assay?

Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...

21 July 2024 9,914 1 View

What are the modern topics to work under bacteriocin and fermentation as PhD student?

As PhD student specialized as food and industrial microbiology need to further research on bacteriocin and fermented food.

16 July 2024 7,394 1 View

How to get a research question in educational administration and management in MPhil?

What are some of the areas to consider in undertaken research in educational administration in MPhil.

14 July 2024 8,394 2 View

How can i elucidate a compound using xrd?

XRD analysis

08 July 2024 977 4 View

How i can calculate mathematically COD concentration if i add 1gr from glucose to 1 L distilled water ?

how i can calculate mathematically COD concentration if i add 1gr from glucose to 1 L distilled water

08 July 2024 9,104 2 View

What is the best method for modeling the transmission and reflection of an electromagnetic wave incident on a magnetic multilayer thin film?

Previously, I successfully modelled the transmission and reflection of electromagnetic waves incident on non-magnetic materials. Please refer to my publications for details: 1- A polynomial model...

05 July 2024 1,396 2 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

In terms of chaos, what is the necessary and sufficient condition for authoritarianism, permanent or temporary, to come to exist and persist?

Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...

28 July 2024 6,515 1 View

What is effective targeted chaos?

Perfect democracy thinking assumes no chaos so no need for independent rule of law system and liberal democracies assume the possibility of normal democratic chaos that can be sorted out by an...

28 July 2024 473 1 View

I want to get updates regarding international law, international trade law, international institutions and human rights etc?

Please send me updates regarding the above-mentioned areas in the question mark?

28 July 2024 5,197 3 View

Malcolm Nobre

Dear Prof. Mohammed Adil Selka

It could be DMSO-induced denaturation. You may want to refer to the below attached papers to understand the effect of DMSO on albumin.

Article Preferential solvatation of human serum albumin in dimethyls...

Article Comparison of DMSO-induced denaturation of hen egg-white lys...

Article Structural Transformation of Bovine Serum Albumin Induced by...

https://www.slas-discovery.org/article/S2472-5552(22)08442-8/pdf#:~:text=In%20the%20present%20study%2C%20we,binding%20properties%20of%20the%20proteins.

Article Protein precipitation and denaturation by dimethyl sulfoxide

The solution to this problem would be to prepare the plant extract in other organic solvents such as methanol or ethyl acetate except DMSO.

Regards,

Mohammed Adil Selka

Dear colleague,

Thanks for your reply.

I had some doubts about the solvent effets on albumin,I'll adjust the protocol using another solvent .

Best regards