Hi all,
I am having trouble deciding if this is cell contamination or something else. I keep seeing this fiber like bodies in my media (not on the same level as my adherent cells). There is no smell or media color change that indicates contamination. I do also see these fibers when i attempt to do a cell count with trypan blue. Has anyone seen something similar? I dont see any movement at all and they dont move when I jiggle the flask gently. I am concerned since I keep seeing these even after I rinse with PBS and place in new media. Is something off with my sterile filter when making my media or is this some type of yeast or bacterial contamination?
Dear Ashley,
It looks to me like fibers from, for example, pulp or a similar material. Check the procedure of your experiment and the handling to see if such a fiber entry is possible at that point.
Jochem
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