Is perfusion neccessary for brain IHC analysis?

Hi, we are generating primary neurons - I was curious if the remainder of the brain could be fixed in PFA and used for IHC analysis without prior perfusion fixation?

21 September 2020 4,358 3 View

I have my cDNA and matching mRNA sequence for the protein that I am eventually looking to synthesis and I cant figure out the right primer for RT?

I have both my cDNA and matching mRNA sequence for the protein that I am eventually looking to synthesis and I cannot, for the life of me, figure out how to select said mRNA in the RT process ?

02 April 2019 3,651 3 View

Do I include the whole primer- amplified length of DNA (PCR) when calculating the concentration of DNA target region within the amplified DNA length?

I have to find the concentration of my target sequence, for which I had no choice but to amplify a 400 bp region of the sequence containing our target sequence (which is only 40 bp). Would I have...

19 October 2016 5,941 3 View

I have questions about remote sensing of oil pollution of the soil.

I have questions a bout remote sensing of oil pollution of the soil,does anyone have any information that can help me?i would really appreciate for any help.

29 February 2016 8,626 7 View

How do I simulate Dugdale Strip model in FEM, for Dynamic cracks using commercially available software like ABAQUS?

Dear all, I am trying to simulate or get the shape of Dugdale plastic Zone for a growing crack near the vicinity of the crack tip. I am able to get enough information about static crack but I fail...

07 October 2014 2,109 3 View

Do control mice behave the same as they did 100 years ago?

Since we have detailed data of some strains of mice, such as C57BL/6, from experiments dating to almost 100 years ago, I wondered - do mice we use in experiments today that are derived from this...

01 January 1970 3,584 1 View

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02 March 2021 3,060 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View