How can I detect a frameshift mutation in both the alleles of a gene induced by my CRISPR-Lentivirus?

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Liu Zhenlong

Are you using the CAS9 / CRISPR Lentivirus to knock out your target gene? If so, you can extract mRNA and use the RT-PCR, then you need do squencing your cDNA of your PCR product. We did some, and it works very well. Hope it can help you! Good luck!

Syed Raza

Thanks Liu, yes I am doing KO exit. And now planing to do the sequencing but before selecting to do clone the product ?