I need to filter about 100 micro litres of my solution with a 0.22um filter according to the procedure. How do I go about it?

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When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

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Khushman Taunk

there are different syringe filters available. u can use the smallest volume one but i think u will have atleast 20-30% loss.

Andrew D Winters

You could use a centrifugal filter.

http://www.emdmillipore.com/US/en/product/Ultrafree%C2%AE-MC-and--CL-Centrifugal-Filter-Units,MM_NF-C7554?isCountryEMD=yes

Hayder Obayes Hashim

according to the protocol you do not need to filtrate this quantity , if you mean the step "4" for sample you can take any volume and filtrate it , then take the required volume which have to be add to the plat .

regard

Tanko Abubakar Sadiq

Thank you all for your contributions.