5000 kDa filter may not work, best way is to use a dialysis membrane, but it is a time consuming process. I have no experience on sephadex column.
To Aditya Arya: sorry for making an unclear question. I mean the membrane of dialysis filtration with the pore size of 5 kDa. I dont know if the dialysis filtration is more effective and faster to remove Na+ than gel filtration method?
To Dr Murugesan Muthu: When we make the solution acidic, I'm afraid that the activity of protein may be changed and precipitate appears up to the decrease of protein solubility in acidic condition.
Thanks Xuannam Truong
Have a look - it may serve your expectation
Protein Desalting Spin Columns from Pierce
http://wolfson.huji.ac.il/purification/PDF/dialysis/PIERCE_Desalting_Spin_Columns.pdf
If you protein is 5K, you may try dialysis experiment to decrease the salt concentration in the protein solution. But you got to choose the right semi-permeable membrane with appropriate pore size.
Good luck!
Hello every body!
To Dr Murugesan Muthu: thank you for your suggestion, but the volume of the spin column is to small. My sample volume is about 30-50 ml.
To Ramanjeet Kaur: thanks, I'm really interested in your answer. Could you please tell me more in details about the concentration of the aqueous protein solution. I mean at what concentration we could start the dialysis? As you said, we should repeat the dialysis circle 2-3 times?
and Jiansheng Huang: Thanks, my protein is about 28k Da. But I'm going to use polyethernsulfone membrane of 5 kDa, is it a good choice for removing Na+ from aqueous protein solution? Could you tell me more about the technique?
I think it is good enough to remove the salt from you protein solution if you use 5kDa membrane. To get rid of more salt, you may repeat 2-3 dialysis cycle.
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biochemical Techniques