Although HRP lives under darkness most of its natural life, what possible biological role Energy transfer from Trp to heme can have? Can it possibly effect Fe reactivity in Compound I or II if exposed to UV?
Without ruling out the possibility of an effect in the unusual presence of UV light to excite Trp residues (Excitation maximum at 280 nm), the efficiency of energy transfer between Trp residues and heme would not be very high because the heme absorbance is weak at the emission maximum of Trp (330-360 nm).
Thanks for your valuable reply. Has any body carried out an activity comparison of Horseradish peroxidase in darkness vs under UV light (280-295 nm) ? I am unable to find such a study.
The destructiveness of UV light and the absorption of the light by the reaction components could add a challenge to this experiment, but if the reaction time and path length are kept short it might be possible to get a rate measurement. You should try it.
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