Proteoglycan in liquid samples was estimated by periodic Schiffs assay method (Mantle and Allen, 1978). Whether the presence of free glycosaminoglycan in the sample will interfere the proteoglycan estimation?. If so there is any other method which is more specific to proteoglycan?
Dear Sir. Concerning your issue about the estimation of proteoglycan in liquid samples . Proteoglycans were separated by high-performance liquid chromatography (HPLC), using two coupled Aquapore columns containing glycerylpropylsilane groups covalently linked to large-pore (50–100 nm) silica spheres. This two-column HPLC system was effective in separating cartilage proteoglycan aggregates and monomers, without altering their biochemical integrity. This system was also effective in resolving small amounts of isotopically labeled proteoglycans synthesized by cultured mammalian cells. The small sample size, short analysis time, and high reproducibility represent improvements in the study of proteoglycans over conventional soft-gel chromatography. I think the following below links and the attached file may help you in your analysis:
http://www.sciencedirect.com/science/article/pii/0003269782901282
https://www.ncbi.nlm.nih.gov/pubmed/8665780
Thanks
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