i want to grow rhizosphere bacteria on nutrient broth and subsequently check their growth through OD. I need a robust protocol that can tell me all the details about conducting this experiment.
Find the attached article, you can grow culture and get final culture suspension shown in yellow marked text, check OD against 0.85 % NaCl blank. Hope that will help you.
You can see the attached file, may be useful for for media preparation.For checking the growth through OD you can take the OD value at 600 nm against the uninoculated control media.