Could someone suggests which MW of FITC-Dextran is used for chemotaxis of bacteria? Also, if I make a FITC-dextran solution in PBS then how long can we store this solution at 4 degree Celsius?
https://www.nature.com/articles/s41598-018-24748-7.pdf
Can anybody say which calcium binding agent (which either fluoroscence or give colours) are use to measure calcium level outside the cell or in extracellular media ?
07 August 2019 5,929 2 View
If photoresist is coated over first alignment marks then how will I do 2nd alignment through MJB4 mask aligner?
07 August 2019 7,515 4 View
I want 1000 bacteria (numbers) to begin my experiments .How will i get these numbers? Any specific methods? please give suggestions.
07 August 2019 3,710 12 View
How to track multiple particles or bacteria in ImageJ? I am using multitracker in imageJ but unable to track many particles and bacteria. please help and give valuable suggestions.
06 July 2019 3,405 9 View
Could anybody say what is the general porosity of PDMS , i mean pore size ? can we use PDMS for DNA run?? one more thing what is the pore size of Whatmann filter paper??
03 April 2019 2,560 2 View
How do make a glycerol stock of bacteria for future use? I need valuable suggestions for that.Please put here and share your experience.
01 February 2019 3,626 3 View
I got a agar stabs of bacterial strains, how will i use for this for my experiment i.e how to subculture or revive from stored strains in agar stab?
01 February 2019 3,040 4 View
Fluorescent intensity of cytosolic GFP of bacterial cell is assumed to be equal to their cell volume? what does it mean?. could anybody explain it why cytosolic GFP? and their intensity...
01 February 2019 7,864 5 View
What is L-Form of Bacteria. How can we make E.coli L-form i mean what is the protocol to make it L-form. How can i maintain this L-form to longer time. If not , from where can we order simple...
08 September 2018 7,854 0 View
Everytime i found different OD data which was fluctuating and sometimes continue increase of OD .Though growth curve and colony under microscope dont tell wheather it is contaminated or not.If...
08 September 2018 461 15 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...
27 February 2021 428 3 View
Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...
24 February 2021 9,839 3 View
Most of the papers have compared different hyperparameters such as layers, initializers, activation function, and optimizer algorithm in the CNN algorithm for detecting network intrusion. This is...
24 February 2021 4,578 4 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...
23 February 2021 6,756 1 View
a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards
22 February 2021 5,988 2 View