Could someone suggests which MW of FITC-Dextran is used for chemotaxis of bacteria? Also, if I make a FITC-dextran solution in PBS then how long can we store this solution at 4 degree Celsius?
https://www.nature.com/articles/s41598-018-24748-7.pdf
Can anybody say which calcium binding agent (which either fluoroscence or give colours) are use to measure calcium level outside the cell or in extracellular media ?
07 August 2019 6,057 2 View
If photoresist is coated over first alignment marks then how will I do 2nd alignment through MJB4 mask aligner?
07 August 2019 7,641 4 View
I want 1000 bacteria (numbers) to begin my experiments .How will i get these numbers? Any specific methods? please give suggestions.
07 August 2019 3,828 12 View
How to track multiple particles or bacteria in ImageJ? I am using multitracker in imageJ but unable to track many particles and bacteria. please help and give valuable suggestions.
06 July 2019 3,598 9 View
Could anybody say what is the general porosity of PDMS , i mean pore size ? can we use PDMS for DNA run?? one more thing what is the pore size of Whatmann filter paper??
03 April 2019 2,710 2 View
I got a agar stabs of bacterial strains, how will i use for this for my experiment i.e how to subculture or revive from stored strains in agar stab?
01 February 2019 3,249 4 View
How do make a glycerol stock of bacteria for future use? I need valuable suggestions for that.Please put here and share your experience.
01 February 2019 3,841 3 View
Fluorescent intensity of cytosolic GFP of bacterial cell is assumed to be equal to their cell volume? what does it mean?. could anybody explain it why cytosolic GFP? and their intensity...
01 February 2019 7,971 5 View
What is L-Form of Bacteria. How can we make E.coli L-form i mean what is the protocol to make it L-form. How can i maintain this L-form to longer time. If not , from where can we order simple...
08 September 2018 8,013 0 View
Everytime i found different OD data which was fluctuating and sometimes continue increase of OD .Though growth curve and colony under microscope dont tell wheather it is contaminated or not.If...
08 September 2018 593 15 View
need to review articles on care giving in dementia and challenges faced
12 August 2024 6,424 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...
30 July 2024 1,412 8 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View