An antibody, also known as an immunoglobulin, is a large Y-shaped protein produced by B-cells that is used by the immune system to identify and... | Contact experts in Antibodies to get answers
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Questions related to Antibodies
Hi RG Community I had input all the appropriate files, however, received an Action halted on error! My ORCA generated .gbw QM Output file was not recognized (please see screenshot attached). I...
16 February 2024 2,602 1 View
I am analyzing the lycopene, flavonoid and phenolic contents of dry fruits compared to their fresh state using the same laboratory procedure. Therefore, I am presenting both results on the same...
14 February 2024 6,828 2 View
We have a Leica CM1520 cryostat which we use disposable blades in. Are there any covers or guards available for purchase to protect ourselves when cleaning up or mounting/removing the blade?
09 February 2024 2,548 0 View
Introduction to polymers
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I am running EMSAs with different DNA probes (ss/ds/stem-loop etc.). I hybridized and purified the probes, and consistently see my hybridized and PAGE-purified stem-loop probes run faster than my...
07 February 2024 8,153 0 View
Hello everyone, I would like to know if anyone has experience in performing RNAseq from lymphocyte suspension (Sorter, MACs, etc.) from fixed and permeabilized samples? I was thinking of sorting...
06 February 2024 2,963 0 View
1. Do we need to separate iNKT cells using certain positive markers, like Vα24 TCRα and CD1d, or negative markers, like CD8, before we can use them in a clinical setting? 2. Should the α-GalCer...
30 January 2024 4,445 0 View
How to perform Western blot for GPI-anchored proteins, and are there any differences in the experimental conditions, particularly in the extraction and processing procedures of protein samples,...
29 January 2024 4,319 0 View
I am working in the field of preparing nanoparticles by the Exploding wire method. Does changing the amount of wire exploding voltage affect the size of nanoparticles?
27 January 2024 7,403 2 View
There is better bonding between the mother and baby at home because there is less interference. There are studies showing better weight gain, calmer babies, better adaption of microbiome. I am...
27 January 2024 1,408 1 View
The Ki67 staining of the Antibody drug conjugate(Antibody-Mc-Vc-PAB-MMAE)treated tumor tissue was stronger than that of the control group, but the tumor tissue treated with the drug was indeed...
25 January 2024 9,574 0 View
acta jure imperii, state sovereign immunity, genocide, war crimes, crimes against humanity, military occupation, property rights, civil tort law, jurisdiction, right to a fair trial.
23 January 2024 7,813 3 View
Standard insulin pellets from Innovative Research of America have lots of variability on the release of insulin. Is there a better system. Would intermittent insulin dosing (i.p.) be better- if...
17 January 2024 3,755 0 View
I tried to detect my recombinant protein using dot blot. After spotting the cell lysate onto the membrane, I let it dry at room temperature for 1 hour, and then proceeded with blocking and...
11 January 2024 4,323 5 View
For flow cytometric analysis of N1 and N2 phenotype of TANs in breast tumor mice, I need to specifically identify them for ordering antibody and is unable to confirm the correct one. If anybody...
10 January 2024 960 0 View
I am running SDS page gels using the mini-PROTEAN tetra vertical tank. Gels ran normally up until about 3/4 of the way down the gel. At this point, the dye changes from blue to yellow and became...
10 January 2024 7,381 3 View
I need to evaluate abdominal adipose tissue hypertrophy in a murine model with metabolic syndrome, with and without treatment and I need to review which is the best objective to measure adipocyte...
10 January 2024 7,240 0 View
How do matter and energy move through Earth's oceans and atmosphere and how is matter and energy transferred by convection within Earth's layers?
10 January 2024 2,542 4 View
RG citation score is frozen. Anyone noticed that with his/her profile?
10 January 2024 4,935 2 View
Hello! The freezing protocol for MSCs I have been performing says to freeze 1 ml of cell suspension. I was told that in order to keep confluence, I must add only 1 ml to the pellet of cells and...
09 January 2024 1,809 2 View
Cellular biology of human
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if cane answer it
08 January 2024 1,369 0 View
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07 January 2024 5,779 1 View
I did an experiment for a positive control phos-tag system to detect phosphorylated AMPK in min6 cells. I incubated min6 cells for 12h with metformin to induce AMPK phosphorylation then check the...
07 January 2024 7,788 1 View